Asante Kotoko hopes of qualifying to the group stage of the CAF Confederation Cup came to an end after losing to FC San Pedro 2-0 in Abidjan.
San Pedro qualified to the group stage of the competition on a 2-1 aggregate.
Zachariassen made four changes to the team that started in the first leg, Alex Didi, Samuel Frimpong, Wahab Adams and Kelvin Andoh were preferred ahead of Ayiku Tetteh, Augustine Okrah, Emmanuel Gyamfi and Songne Yacouba.
A first half strike from Soumaro Cheick and a late goal by Tidjae Diomande helped FC San Pedro to secure a 2-0 home win over Asante Kotoko at the Felix Houphouet Boigny Stadium.
Soumaro Cheick unsettled Kotoko few minutes into the game by giving the home side the lead.
San Pedro dominated the Ghana Premier League heavyweights to end of the first half in a 1-0 lead.
Kjetil Zachariassen made few changes in the second half by introducing Emmanuel Gyamfi and Augustine Okrah for Didi Alexis and Collins Ameyaw.
Kotoko had few chances to level matters but Kelvin Andoh and Richard Arthur fluffed them over the bar.
The home side got the needed winner in the 96th minute when Tidjae Diomande finished off a good move in the penalty box to double his side lead.
Asante Kotoko were reduced to 10-men prior to the full time whistle after Agyemang Badu was giving his marching orders after receiving his second caution in the game.
FC San Pedro maintained their lead till the blast of the final whistle.